Publications

Norris, A. L. and Serpersu, E. H. (2010) Coenzyme A Interactions with the Aminoglycoside Acetyltransferase (3)-IIIb and the Thermodynamics of a Ternary System. Biochemistry 49,4036–4042.

Norris, A. L., Özen, C., and Serpersu, E. H. (2010) Thermodynamics and Kinetics of Antibiotic Association with the Aminoglycoside Acetyltransferase (3)-IIIb: A Resistance Causing Enzyme. Biochemistry 49,4027-4035.

Serpersu, E. H., Özen, C., Norris, A. L. Steren, C. and Whittemore, N. (2010) Backbone Resonance Assignments of a Promiscuous Aminoglycoside Antibiotic Resistance Enzyme; the Aminoglycoside Phosphotransferase(3′)-IIIa. Biomol. NMR Assign. 4,9-12.

Norris, A. D. and Serpersu, E. H. (2009) NMR Detected Hydrogen-Deuterium Exchange Reveals Differential Dynamics of Antibiotic and Nucleotide Bound Aminoglycoside Phosphotransferase 3′-IIIa. J. Am. Chem. Soc. 131,8587-8594.

Wu, Lingzhi and Serpersu, E. H. (2009) Deciphering Interactions of the Aminoglycoside Phosphotransferase(3′)-IIIa with its Ligands. Biopolymers 91,801-809.

Özen, C., Norris, A. L., Land, M., Tjioe, E., and Serpersu, E. H. (2008) Detection of Specific Solvent Rearrangement Regions of an Enzyme: NMR and ITC Studies with Aminoglycoside Phosphotransferase(3′)-IIIa. Biochemistry, 47,40-49.

Serpersu, E., H., Özen, C., and Wright, E. (2008) Studies of enzymes that cause resistance to aminoglycoside antibiotics. Methods in Molecular Medicine ( New Antibiotic Targets) 142, 261-271.

Özen, C., Malek, J. M., and Serpersu, E. H. (2006). Dissection of Aminoglycoside-Enzyme Interactions: A Calorimetric and NMR Study of Noemycin B Binding to the Aminoglycoside Phosphotransferase(3')-IIIa. J. Am. Chem. Soc. 128, 15248-15254.

Serpersu, E., H., Özen, C., and Wright, E. (2006) Thermodynamic Studies of Aminoglycoside Antibiotic-Enzyme Interactions. Turkish J. Biochem.31,79-85.

Wright, E. and Serpersu, E. H. (2006) Molecular Determinants of Affinity for Aminoglycoside Binding to the Aminoglycoside Nucleotidyltransferase(2″)-Ia. Biochemistry 45,10243-10250.

Wright, E. and Serpersu, E. H. (2005) Enzyme–Substrate Interactions with an Antibiotic Resistance Enzyme: Aminoglycoside Nucleotidyltransferase(2″)-Ia Characterized by Kinetic and Thermodynamic Methods. Biochemistry 44, 11581-11591.

Welch, K. T., Virga, K. G., Brown, C. L., Wright, E., Lee R. E., and Serpersu, E. H. (2005) Discovery of non-carbohydrate inhibitors of aminoglycoside-modifying enzymes. Bioorg. Med. Chem. 13,6252-6263.

Whittemore,N. A., Mishra, R., Kheterpal, I., Williams,A. D., Wetzel, R., and Serpersu, E. H. (2005) Hydrogen–Deuterium (H/D) Exchange Mapping of Aβ_1–40 Amyloid Fibril Secondary Structure Using NMR Spectroscopy. Biochemistry 44,4434 – 4441.

Özen, C. and Serpersu, E. H. (2004) Thermodynamics of aminoglycoside binding to aminoglycoside-3′-phosphotransferase IIIa studied by isothermal titration calorimetry. Biochemistry 43,14667-14675.

Götmar, G., Özen, C., Serpersu, E. H., and Guiochon, G. (2004) Isothermal Microcalorimetric Study of the pH-Dependence of the Interactions Between a Cellulase and α-Blocker. J. Chromatogr. A 1046,49-53.

Wright, E. and Serpersu, E. H. (2004) Isolation of Aminoglycoside Nucleotidyltransferase (2″)-Ia from Inclusion Bodies as Active, Monomeric Enzyme. Protein Express. Purif. 35,373-380.

Mayasundari, A., Whittemore, N. A., Serpersu, E. H., and Peterson, C. B. (2004) The solution structure of the N-terminal domain of human plasma vitronectin: Proximal binding sites that regulate fibrinolysis and cell migration. J. Biol. Chem. 279, 29359 - 29366.

Horn, N. A., Hurst, G. B., Mayasundari,, A., Neil A. Whittemore, N. A., Serpersu, E. H., and Cynthia B. Peterson, C. B. (2004) Assignment of the Four Disulfides in the N-terminal Somatomedin B Domain of Native Vitronectin Isolated from Human Plasma. J. Biol. Chem. 279,35867 – 35878.

Owston, A. M., and Serpersu, E. H. (2002) Cloning, overexpression and purification of aminoglycoside antibiotic 3-acetyltransferase-IIIb: Conformational studies with bound substrates. Biochemistry 41,10764-10770.

Gupta, R. K., Dobritsa, S. V., Stiles, C. A., Essington, M. E., Liu, Z., Chen, C-H., Serpersu, E. H., and Mullin, B. C. (2002) Metallohistins: A new class of plant metal-binding proteins. J. Prot. Chem. 21,529-536.

Ekman, D. R., DiGiammarino, E. L., Wright, E., Witter, E. D., Serpersu, E. H. (2001) Cloning overexpression and purification of aminoglycoside antibiotic nucleotidyl transferase (2")-Ia: Conformational Studies with bound substrates. Biochemistry 40,7017-7024.

Xu, Y., Xu, D., Crawford, O. H., Einstein, J. R., and Serpersu, E (2000) Protein structure determination using protein threading and sparse NMR data. Proceedings of The Fourth Annual International Conference on Computational Molecular Biology, 299 - 307.

Serpersu E. H., Cox, J. R., DiGiammarino, E. L., Mohler, M. L.,Ekman, D. R.,Akal-Strader, A. Owston, M. (2000) Conformations of Antibiotics in Active Sites of Aminoglycoside-Detoxifying Enzymes. Cell Biochem. & Biophys. 33,309-321.

Cox, J. R., Ekman, D. R., DiGiammarino, E. L., Akal-Strader, A., and Serpersu, E. H. (2000) Aminoglycoside Antibiotics Bound to Aminoglycoside-Detoxifying Enzymes and RNA Adopt Similar Conformations. Cell Biochem. & Biophys. 33,297-308.

Antolovic, R., Hamer, E., Serpersu, E. H., Kost, H., Linnertz, H., Kovarik, Z, Schoner, W.(1999) Affinity labeling with MgATP Analogues reveals coexisting Na⁺, and K⁺ forms of the a-subunits of Na⁺/K⁺ ATPase. Eur. J. Biochem. 261,181-189.

Ismailov, I. I., Shlyonsky, V. G., Serpersu, E. H., Fuller, C. M., Cheung, H. C., Muccio, D., Berdiev, B. K., & Benod, D. J. (1999) Peptide inhibition of EnaC. Biochemistry 38,354-363.

DiGiammarino, E. L., Draker, K., Wright, G. D., and Serpersu, E. H. (1998). Solution Studies of Isepamicin and conformational comparisons between isepamicin and butirosin A when bound to an aminoglycoside 6N-N-acetyltransferase determined by NMR spectroscopy. Biochemistry 37,3638-3644

Murray, M. B., Tadaki, D. K., Campion, S. R., Lamerdin, J. A., Serpersu, E. H.,Bradrick, T. D. & Niyogi, S. K. (1998). Structure-function analysis of a conserved aromatic cluster in the N-terminal domain of human epidermal growth factor. Prot. Eng. 11,1041-1050.

Mohler, L. M., Cox, J.R., Serpersu, E. H. (1998) Aminoglycoside phosphotransferase(3N)-IIIa (APH(3N)-IIIa)-bound conformation of the aminoglycoside lividomycin A characterized by NMR. Carbohydr. Lett. 3,17-24.

Chen, Y-R., Larimer, F. W., Serpersu, E. H., & Hartman, F. C. (1998) Identification of a Catalytic Aspartyl Residue of D-Ribulose 5-Phosphate 3-Epimerase by Site Directed Mutagenesis. J. Biol. Chem. 274,2132-2136

Gibson, A. D., Lamerdin, J. A., Zhuang, P., Serpersu, E. H.,& Peterson, C. B. (1998) Orientation of Heparin Binding Sites in Native Vitronectin: Analyses of Ligand Binding to the Primary Glycoseaminoglycan-binding Site Indicate that Putative Secondary Sites are not Functional. J. Biol. Chem. 274, 6432-6442.