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University of Tennessee Mycology Lab

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Welcome! » Fungal Laboratory Techniques


Fungal Laboratory Techniques

Karen W. Hughes

  1. PRELIMINARIES

    1. Safety

    2. Recording Data

      • Keeping a lab notebook
      • Labeling samples

    3. Calculations and preparation of solutions

  2. Field preservation of samples for DNA extraction

  3. DNA-BASED TECHNIQUES

    1. What DNA sequence should I use for evolutionary comparisons?

    2. CTAB-based DNA extraction

    3. Concentrating DNA Samples

    4. Agarose gels

    5. Polymerase chain reaction – PCR

    6. Restriction digests

    7. DNA Sequencing (A graphical presentation of Automated Sequencing)

    8. Photographing gels with the Polaroid camera

  4. APPENDIX

    1. Common solutions
      • Tris and EDTA solutions, TE buffer
      • Salt solutions
      • Solutions for agarose electrophoresis
      • Molecular weight markers
      • Southern Blot solutions

    2. Molecular weight standards for gel electrophoresis


Note:  If you wish to cite these techniques, one of the following papers can be cited:

Hughes, Karen W. and Ronald H. Petersen. (1997) Relationships between Omphalotus species based on RFLP patterns of the ribosomal ITS1-5.8S-ITS2 region. Plant Syst. Evol. 211:213-237

Hughes, K.W., T. Toyahara and R.H.Petersen (1998) Disjunct populations of Pleurotopsis longinqua. Mycologia 90 (4):595-600

Comments, corrections, suggestions?   E-mail khughes@utk.edu