Plant Fluorescent Expression Vectors
We have created a series of plant expression vectors that allow translational fusion to a variety of fluorescent proteins (FPs).
Excitation and emission spectra of the proteins can be found in these PDF files (graphs generated with data from the Tsien lab). Cerulean has the same spectrum as CFP. Please compare these spectra with the filter sets available to you before ordering something that doesn't work on your scope. If you're planning to do dual labeling, be aware that there is significant spectral overlap between the different FPs. Depending on your filter set (and relative expression levels) CFP/Cerulean + YFP or GFP + mCherry are usually safe combinations. As always, it is best to run a series of controls to check for cross-talk between the channels.
All plasmids have been created by Kevin Allen, except for pAN581, which was made by Dr. Xue Cai.