LOCUS vac-rb 10371 bp DNA circular 18-OCT-2007 DEFINITION pAN611. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|443790721| COMMENT VNTDBDATE|460549219| COMMENT VNTNAME|vac-rb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 9045..9071 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(9155..9406) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(9417..9968) /gene="BAR" /vntifkey="60" CDS complement(9417..9968) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9974..10354) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2767..2790 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3852..4852) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(7255..7536) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7826..8620) /gene="aadA" /vntifkey="60" CDS complement(7826..8620) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" primer_bind 2288..2307 /vntifkey="28" /label=seq-NOS3-R primer_bind 673..696 /vntifkey="28" /label=35S\primer promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 2278..2527 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" CDS 788..1540 /vntifkey="4" /label=gammaTIP /note="Arabidopsis gamma TIP ORF (At2g36830)" CDS 1547..2257 /vntifkey="4" /label=mCherry BASE COUNT 2476 a 2872 c 2810 g 2213 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 actcgagatg ccgatcagaa acatcgccat tggccgtcca gatgaagcca cccgtcccga 841 tgccttaaag gcggcgttgg ctgagttcat ttcaactttg atctttgtcg tcgccggttc 901 aggctctggc atggctttca acaagctcac tgaaaacgga gccaccactc cttctggtct 961 cgtagctgct gcagtggctc atgcctttgg actcttcgtc gctgtctcag ttggtgccaa 1021 catctctggt ggacacgtta accctgccgt cactttcggt gctttcattg gtggtaacat 1081 cactctcctc cgtggtatcc tctactggat tgctcagctt ctcggctccg tcgtcgcttg 1141 cctcatcctt aaattcgcca ccggtggctt ggctgtgccg gcttttggtc tctctgctgg 1201 agtaggagtg ttgaacgctt tcgttttcga gatcgtgatg acattcgggc ttgtttacac 1261 cgtctacgct acagccattg accccaaaaa cgggagtctt ggaacaattg ctcccatcgc 1321 aatcggtttc attgttggag ccaacatctt agctggagga gctttctctg gagcctccat 1381 gaatcccgcc gtggctttcg gaccagcggt ggtgagctgg acatggacca accactgggt 1441 ctactgggcc ggacctctcg tcggcggtgg aatcgctgga ctcatctacg aagttttctt 1501 catcaacacc acacacgagc agctcccaac cacagactac ggatccatgg tgagcaaggg 1561 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg 1621 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg 1681 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat 1741 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc 1801 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga 1861 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta 1921 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac 1981 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga 2041 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac 2101 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga 2161 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg 2221 ccactccacc ggcggcatgg acgagctgta caagtaaagc ggccgcccgg ctgcagttca 2281 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc 2341 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta 2401 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa 2461 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta 2521 gatcgggaat tcgatatcaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga 2581 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 2641 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 2701 atgctagagc agcttgagct tggatcagat tgtcgtttcc cgccttcagt ttaaactatc 2761 agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt attagaataa 2821 cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg tgcatgccaa 2881 ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg ctatagtgca 2941 gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca caagtcctaa 3001 gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc gtgttttagt 3061 cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa caagagcgcc 3121 gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt gaccaaccaa 3181 cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat caccggcacc 3241 aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg cgacgttgtg 3301 acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga cattgccgag 3361 cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc cgacaccacc 3421 acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt cgagcgttcc 3481 ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg cgtgaagttt 3541 ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct gatcgaccag 3601 gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc gaccctgtac 3661 cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg cggtgccttc 3721 cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga acgccaagag 3781 gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt accgaagaga 3841 tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac gtctcaaccg 3901 tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc tggccggcca 3961 gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta tttgagtaaa 4021 acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa caaatacgca 4081 aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag gcaagacgac 4141 catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc tgttagtcga 4201 ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc aaccgctaac 4261 cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg gccggcgcga 4321 cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg cgatcaaggc 4381 agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg ccaccgccga 4441 cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac aagcggcctt 4501 tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg aggcgctggc 4561 cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct acccaggcac 4621 tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg cccgcgaggt 4681 ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg taaagagaaa 4741 atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag cagcaaggct 4801 gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt tcagttgccg 4861 gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac cattaccgag 4921 ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat aaatgagtag 4981 atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag gcaccgacgc 5041 cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg gctgggttgt 5101 ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt gacggtcgca 5161 aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga 5221 aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt 5281 ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc 5341 cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct 5401 atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga 5461 agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg 5521 tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg 5581 tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc 5641 gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa 5701 agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc 5761 agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga 5821 ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc 5881 tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc 5941 accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc 6001 gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca 6061 gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc 6121 tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct 6181 accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag 6241 ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt 6301 acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg aacccaaagc 6361 cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa aaaggcgatt 6421 tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg gcctgtgcat 6481 aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc 6541 gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg 6601 ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc 6661 gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc 6721 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga 6781 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag 6841 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac 6901 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 6961 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 7021 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 7081 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 7141 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 7201 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 7261 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 7321 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 7381 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7441 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7501 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7561 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7621 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7681 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7741 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7801 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta 7861 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt 7921 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc 7981 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc 8041 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa 8101 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca 8161 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat 8221 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca 8281 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat 8341 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg 8401 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg 8461 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca 8521 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga 8581 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt 8641 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact 8701 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa 8761 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc 8821 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa 8881 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc 8941 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt 9001 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag gatatattgt 9061 ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt tttaatgtac 9121 tgaattaacg ccgaattaat tcgagctcgg atctgataat ttatttgaaa attcataaga 9181 aaagcaaacg ttacatgaat tgatgaaaca atacaaagac agataaagcc acgcacattt 9241 aggatattgg ccgagattac tgaatattga gtaagatcac ggaatttctg acaggagcat 9301 gtcttcaatt cagcccaaat ggcagttgaa atactcaaac cgccccatat gcaggagcgg 9361 atcattcatt gtttgtttgg ttgcctttgc caacatggga gtccaagatt ctgcagttag 9421 atctcggtga cgggcaggac cggacggggc ggtaccggca ggctgaagtc cagctgccag 9481 aaacccacgt catgccagtt cccgtgcttg aagccggccg cccgcagcat gccgcggggg 9541 gcatatccga gcgcctcgtg catgcgcacg ctcgggtcgt tgggcagccc gatgacagcg 9601 accacgctct tgaagccctg tgcctccagg gacttcagca ggtgggtgta gagcgtggag 9661 cccagtcccg tccgctggtg gcggggggag acgtacacgg tcgactcggc cgtccagtcg 9721 taggcgttgc gtgccttcca ggggcccgcg taggcgatgc cggcgacctc gccgtccacc 9781 tcggcgacga gccagggata gcgctcccgc agacggacga ggtcgtccgt ccactcctgc 9841 ggttcctgcg gctcggtacg gaagttgacc gtgcttgtct cgatgtagtg gttgacgatg 9901 gtgcagaccg ccggcatgtc cgcctcggtg gcacggcgga tgtcggccgg gcgtcgttct 9961 gggctcatcg attcgatttg gtgtatcgag attggttatg aaattcagat gctagtgtaa 10021 tgtattggta atttgggaag atataatagg aagcaaggct atttatccat ttctgaaaag 10081 gcgaaatggc gtcaccgcga gcgtcacgcg cattccgttc ttgctgtaaa gcgttgtttg 10141 gtacactttt gactagcgag gcttggcgtg tcagcgtatc tattcaaaag tcgttaatgg 10201 ctgcggatca agaaaaagtt ggaatagaaa cagaataccc gcgaaattca ggcccggttg 10261 ccatgtccta cacgccgaaa taaacgacca aattagtaga aaaataaaaa ctagctcaga 10321 tacttacgtc acgtcttgcg cactgatttg aaaaatctca gaattcgagc t //