LOCUS G-gb 9916 bp DNA circular 18-OCT-2007 DEFINITION pAN232. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|446466209| COMMENT VNTDBDATE|460546749| COMMENT VNTNAME|G-gb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 8590..8616 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(8700..8951) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(8962..9513) /gene="BAR" /vntifkey="60" CDS complement(8962..9513) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9519..9899) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2312..2335 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3397..4397) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(6800..7081) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7371..8165) /gene="aadA" /vntifkey="60" CDS complement(7371..8165) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" CDS 762..1056 /vntifkey="4" /label=SM1\(t-TM-s) /note="cytoplasmic tail + TM domain + lumenal stalk from soybean mannosidase" promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 1823..2072 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" misc_feature 762..848 /vntifkey="21" /label=cytoplasmic\tail misc_feature 849..896 /vntifkey="21" /label=TransMembrane\domain CDS 1083..1802 /vntifkey="4" /label=GFP /note="GFP coding region" BASE COUNT 2422 a 2722 c 2687 g 2085 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc tttgtttggg aatggcgaga gggagcagat 781 cagtgggtag cagcagcagc aaatggaggt actgcaaccc ttcctattac ttgaagcgcc 841 caaagcgtct tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc 901 gtcaaactct cgtcagagag caccaggttg aaatttctga gctgcagaaa gaagtgactg 961 atttgaaaaa tttggtggat gatttaaata acaaacaagg tggtacctct gggaagactg 1021 acttggggag aaaagctacc aagtccagta aagacgtctc gaggaccggt cccgggggat 1081 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg 1141 acggcgacgt gaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct 1201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca 1261 ccctcgtgac caccttcacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga 1321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct 1381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc 1441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc 1501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga 1561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg 1621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc 1681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg 1741 tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag ctgtacaagt 1801 aaagcggccg cccggctgca gttcaaacat ttggcaataa agtttcttaa gattgaatcc 1861 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat 1921 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca 1981 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc 2041 gcgcgcggtg tcatctatgt tactagatcg ggaattcgat atcaagcttg gcactggccg 2101 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 2161 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 2221 aacagttgcg cagcctgaat ggcgaatgct agagcagctt gagcttggat cagattgtcg 2281 tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa 2341 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt 2401 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat 2461 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga 2521 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg 2581 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag 2641 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac 2701 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct 2761 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga 2821 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac 2881 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct 2941 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc 3001 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc 3061 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc 3121 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct 3181 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac 3241 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc 3301 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac 3361 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc 3421 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat 3481 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta 3541 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg 3601 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc 3661 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg 3721 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg 3781 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg 3841 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg 3901 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc 3961 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca 4021 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg 4081 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca 4141 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac 4201 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca 4261 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg 4321 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat 4381 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt 4441 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta 4501 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa 4561 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt 4621 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc 4681 ccgaggaatc ggcgtgacgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg 4741 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag 4801 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc 4861 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa 4921 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg 4981 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac 5041 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg 5101 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg 5161 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag 5221 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg 5281 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg 5341 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg 5401 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc 5461 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc 5521 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc 5581 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg 5641 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag 5701 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt 5761 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa 5821 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg 5881 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact 5941 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact 6001 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa 6061 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc 6121 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga 6181 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg 6241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 6301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 6361 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 6421 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 6481 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 6541 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 6601 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 6661 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6721 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6781 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6841 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6901 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6961 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 7021 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 7081 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 7141 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 7201 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 7261 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 7321 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat 7381 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca 7441 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag 7501 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact 7561 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc 7621 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg 7681 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat 7741 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga 7801 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca 7861 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca 7921 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg 7981 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa 8041 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta 8101 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta 8161 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt 8221 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa 8281 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt 8341 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc 8401 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat 8461 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac 8521 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg 8581 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa 8641 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcgag ctcggatctg 8701 ataatttatt tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca 8761 aagacagata aagccacgca catttaggat attggccgag attactgaat attgagtaag 8821 atcacggaat ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact 8881 caaaccgccc catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca 8941 tgggagtcca agattctgca gttagatctc ggtgacgggc aggaccggac ggggcggtac 9001 cggcaggctg aagtccagct gccagaaacc cacgtcatgc cagttcccgt gcttgaagcc 9061 ggccgcccgc agcatgccgc ggggggcata tccgagcgcc tcgtgcatgc gcacgctcgg 9121 gtcgttgggc agcccgatga cagcgaccac gctcttgaag ccctgtgcct ccagggactt 9181 cagcaggtgg gtgtagagcg tggagcccag tcccgtccgc tggtggcggg gggagacgta 9241 cacggtcgac tcggccgtcc agtcgtaggc gttgcgtgcc ttccaggggc ccgcgtaggc 9301 gatgccggcg acctcgccgt ccacctcggc gacgagccag ggatagcgct cccgcagacg 9361 gacgaggtcg tccgtccact cctgcggttc ctgcggctcg gtacggaagt tgaccgtgct 9421 tgtctcgatg tagtggttga cgatggtgca gaccgccggc atgtccgcct cggtggcacg 9481 gcggatgtcg gccgggcgtc gttctgggct catcgattcg atttggtgta tcgagattgg 9541 ttatgaaatt cagatgctag tgtaatgtat tggtaatttg ggaagatata ataggaagca 9601 aggctattta tccatttctg aaaaggcgaa atggcgtcac cgcgagcgtc acgcgcattc 9661 cgttcttgct gtaaagcgtt gtttggtaca cttttgacta gcgaggcttg gcgtgtcagc 9721 gtatctattc aaaagtcgtt aatggctgcg gatcaagaaa aagttggaat agaaacagaa 9781 tacccgcgaa attcaggccc ggttgccatg tcctacacgc cgaaataaac gaccaaatta 9841 gtagaaaaat aaaaactagc tcagatactt acgtcacgtc ttgcgcactg atttgaaaaa 9901 tctcagaatt cgagct //