LOCUS G-cb 9772 bp DNA circular 28-NOV-2007 DEFINITION pAN230. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|446466317| COMMENT VNTDBDATE|464184719| COMMENT VNTNAME|G-cb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 8446..8472 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(8556..8807) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(8818..9369) /gene="BAR" /vntifkey="60" CDS complement(8818..9369) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9375..9755) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2168..2191 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3253..4253) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(6656..6937) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7227..8021) /gene="aadA" /vntifkey="60" CDS complement(7227..8021) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" CDS 762..911 /vntifkey="4" /label=SM1\(t-TM) /note="cytoplasmic tail + TM domain of soybean mannosidase" promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 1679..1928 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" primer_bind 1412..1430 /vntifkey="28" /label=SEQ-CFP-F1 /note="primer is specific for CFP" primer_bind complement(1131..1148) /vntifkey="28" /label=SEQ-CFP-R1 /note="primer is specific for CFP" CDS 939..1658 /vntifkey="4" /label=ECFP /note="ECFP coding sequence" BASE COUNT 2369 a 2703 c 2649 g 2051 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc tttgtttggg aatggctagc gggagcagat 781 cagtgggtag cagcagcagc aaatggaggt actgcaaccc tactagttac ttgaagcgcc 841 caaagcgtct tgctctgctc ttcatcgttt tcgtttgtgt ctctttcgtt ttctgggacc 901 gtcaaactga cgtctcgagg accggtcccg ggggatccat ggtgagcaag ggcgaggagc 961 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 1021 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 1081 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctggg 1141 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 1201 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 1261 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 1321 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacatca 1381 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 1441 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 1501 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 1561 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 1621 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcccg gctgcagttc 1681 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat 1741 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt 1801 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga 1861 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact 1921 agatcgggaa ttcgatatca agcttggcac tggccgtcgt tttacaacgt cgtgactggg 1981 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 2041 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 2101 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat 2161 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata 2221 acggatattt aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtgcatgcca 2281 accacagggt tcccctcggg atcaaagtac tttgatccaa cccctccgct gctatagtgc 2341 agtcggcttc tgacgttcag tgcagccgtc ttctgaaaac gacatgtcgc acaagtccta 2401 agttacgcga caggctgccg ccctgccctt ttcctggcgt tttcttgtcg cgtgttttag 2461 tcgcataaag tagaatactt gcgactagaa ccggagacat tacgccatga acaagagcgc 2521 cgccgctggc ctgctgggct atgcccgcgt cagcaccgac gaccaggact tgaccaacca 2581 acgggccgaa ctgcacgcgg ccggctgcac caagctgttt tccgagaaga tcaccggcac 2641 caggcgcgac cgcccggagc tggccaggat gcttgaccac ctacgccctg gcgacgttgt 2701 gacagtgacc aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga 2761 gcgcatccag gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac 2821 cacgccggcc ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc 2881 cctaatcatc gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt 2941 tggcccccgc cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca 3001 ggaaggccgc accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta 3061 ccgcgcactt gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt 3121 ccgtgaggac gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga 3181 ggaacaagca tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag 3241 atcgaggcgg agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc 3301 gtgcggctgc atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc 3361 agcttggccg ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa 3421 aacagcttgc gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc 3481 aaggggaacg catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga 3541 ccatcgcaac ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg 3601 attccgatcc ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa 3661 ccgttgtcgg catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg 3721 acttcgtagt gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg 3781 cagccgactt cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg 3841 acctggtgga gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct 3901 ttgtcgtgtc gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg 3961 ccgggtacga gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca 4021 ctgccgccgc cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg 4081 tccaggcgct ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa 4141 aatgagcaaa agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc 4201 tgcaacgttg gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc 4261 ggcggaggat cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga 4321 gctgctatct gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta 4381 gatgaatttt agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg 4441 ccgtggaatg ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg 4501 tctgccggcc ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc 4561 aaaccatccg gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg 4621 aaggccgcgc aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg 4681 tggcaagcgg ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg 4741 ccgtcgatta ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc 4801 tatgacgtgg gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg 4861 aagcgtgacc gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag 4921 gtttccgcag ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg 4981 gtttcccatc taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc 5041 cgcgtgttcc gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga 5101 aagcagaaag acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg 5161 cagcgtacga agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg 5221 attagccgct acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag 5281 ctagctgatt ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt 5341 caccccgatt actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc 5401 cgcgccgcag gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc 5461 agcgccggag agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac 5521 ctgccggagt acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc 5581 taccgcaacc tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta 5641 gggcaaattg ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg 5701 tacattggga acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag 5761 ccgtacattg ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat 5821 ttttccgcct aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca 5881 taactgtctg gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg 5941 cgctccctac gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg 6001 gctggcctac ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac 6061 cgccggcgcc cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 6121 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 6181 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 6241 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 6301 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 6361 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 6421 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 6481 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 6541 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 6601 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 6661 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 6721 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 6781 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 6841 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 6901 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6961 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 7021 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 7081 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 7141 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 7201 gggattttgg tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt 7261 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct 7321 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc 7381 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg 7441 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa 7501 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc 7561 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta 7621 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac 7681 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca 7741 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt 7801 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag 7861 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc 7921 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg 7981 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt 8041 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac 8101 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa 8161 agttggcgta taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag 8221 caacgctctg tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa 8281 acccggcagc ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg 8341 ccttacaacg gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg 8401 tgattttgtg ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg 8461 tggtgtaaac aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta 8521 ctgaattaac gccgaattaa ttcgagctcg gatctgataa tttatttgaa aattcataag 8581 aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga cagataaagc cacgcacatt 8641 taggatattg gccgagatta ctgaatattg agtaagatca cggaatttct gacaggagca 8701 tgtcttcaat tcagcccaaa tggcagttga aatactcaaa ccgccccata tgcaggagcg 8761 gatcattcat tgtttgtttg gttgcctttg ccaacatggg agtccaagat tctgcagtta 8821 gatctcggtg acgggcagga ccggacgggg cggtaccggc aggctgaagt ccagctgcca 8881 gaaacccacg tcatgccagt tcccgtgctt gaagccggcc gcccgcagca tgccgcgggg 8941 ggcatatccg agcgcctcgt gcatgcgcac gctcgggtcg ttgggcagcc cgatgacagc 9001 gaccacgctc ttgaagccct gtgcctccag ggacttcagc aggtgggtgt agagcgtgga 9061 gcccagtccc gtccgctggt ggcgggggga gacgtacacg gtcgactcgg ccgtccagtc 9121 gtaggcgttg cgtgccttcc aggggcccgc gtaggcgatg ccggcgacct cgccgtccac 9181 ctcggcgacg agccagggat agcgctcccg cagacggacg aggtcgtccg tccactcctg 9241 cggttcctgc ggctcggtac ggaagttgac cgtgcttgtc tcgatgtagt ggttgacgat 9301 ggtgcagacc gccggcatgt ccgcctcggt ggcacggcgg atgtcggccg ggcgtcgttc 9361 tgggctcatc gattcgattt ggtgtatcga gattggttat gaaattcaga tgctagtgta 9421 atgtattggt aatttgggaa gatataatag gaagcaaggc tatttatcca tttctgaaaa 9481 ggcgaaatgg cgtcaccgcg agcgtcacgc gcattccgtt cttgctgtaa agcgttgttt 9541 ggtacacttt tgactagcga ggcttggcgt gtcagcgtat ctattcaaaa gtcgttaatg 9601 gctgcggatc aagaaaaagt tggaatagaa acagaatacc cgcgaaattc aggcccggtt 9661 gccatgtcct acacgccgaa ataaacgacc aaattagtag aaaaataaaa actagctcag 9721 atacttacgt cacgtcttgc gcactgattt gaaaaatctc agaattcgag ct //