LOCUS ER-cb 9719 bp DNA circular 11-OCT-2007 DEFINITION pAN119. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.informaxinc.com/ COMMENT VNTDATE|446465989| COMMENT VNTDBDATE|459947308| COMMENT VNTNAME|ER-cb| COMMENT VNTAUTHORNAME|NebenfŸhr Lab| COMMENT VNTAUTHORTEL|(865) 974-9201| COMMENT VNTAUTHORWWW|http://botany1.bio.utk.edu| COMMENT VNTAUTHORAD1|Univeristy of Tennessee| COMMENT VNTAUTHORAD2|Department of Botany| COMMENT VNTAUTHORAD3|1406 Circle Dr| COMMENT VNTAUTHORAD4|Knoxville, TN 37996-1100| FEATURES Location/Qualifiers repeat_region 8393..8419 /vntifkey="34" /label=LB /note="left border repeat from C58 T-DNA" polyA_signal complement(8503..8754) /vntifkey="25" /label=PolyA_Signal_1 /note="from mannopine synthase" gene complement(8765..9316) /gene="BAR" /vntifkey="60" CDS complement(8765..9316) /gene="BAR" /codon_start=1 /product="phosphinothricin acetyl transferase" /protein_id="AAP78910.1" /db_xref="GI:32265028" /vntifkey="4" /label=BAR-R /note="basta resistance protein" promoter complement(9322..9702) /vntifkey="30" /label=Promoter\(bar) /note="mannopine synthase promoter" repeat_region 2115..2138 /vntifkey="34" /label=RB /note="right border repeat from C58 T-DNA" rep_origin complement(3200..4200) /vntifkey="33" /label=Rep_Origin_1 /note="pVS1" rep_origin complement(6603..6884) /vntifkey="33" /label=Rep_Origin_2 /note="pBR325" gene complement(7174..7968) /gene="aadA" /vntifkey="60" CDS complement(7174..7968) /gene="aadA" /function="kanamycin resistance" /codon_start=1 /product="aminoglycoside phosphotransferase" /protein_id="AAP78911.1" /db_xref="GI:32265029" /vntifkey="4" /label=KAN-R /note="aadA" promoter 10..736 /vntifkey="29" /label=d35S /note="double 35S promoter" terminator 1625..1874 /vntifkey="43" /label=nos\3' /note="terminator from nopaline synthase gene" primer_bind 672..695 /vntifkey="28" /label=35S\primer sig_peptide 788..856 /vntifkey="94" /label=signal\peptide CDS 788..1618 /vntifkey="4" /label=ER-CFP /note="WAK2-signal pipetid+CFP+HDEL" BASE COUNT 2369 a 2681 c 2634 g 2035 t ORIGIN 1 ccctacccct actccaaaaa tgtcaaagat acagtctcag aagaccaaag ggctattgag 61 acttttcaac aaagggtaat ttcgggaaac ctcctcggat tccattgccc agctatctgt 121 cacttcatcg aaaggacagt agaaaaggaa ggtggctcct acaaatgcca tcattgcgat 181 aaaggaaagg ctatcattca agatgcctct gccgacagtg gtcccaaaga tggaccccca 241 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat 301 tgatgtgaca tctccactga cgtaagggat gacgcacaat cccaccccta ctccaaaaat 361 gtcaaagata cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt 421 tcgggaaacc tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta 481 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa 541 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa 601 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac 661 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt 721 tcatttcatt tggagaggac agcccagatc aactagtctt aagtccggag ctagctctag 781 agagaagatg aaggtacagg agggtttgtt cgtggtggct gttttctacc ttgcttatac 841 gcagctagtc aaggggcaac ctcgcaagga gtgcggatcc atggtgagca agggcgagga 901 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa 961 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt 1021 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg 1081 gggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc 1141 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta 1201 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa 1261 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat 1321 cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa 1381 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac 1441 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc 1501 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc 1561 cgccgggatc actctcggca tggacgagct gtacaagaga tctcatgacg agctgtaact 1621 gcagttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga 1681 tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca 1741 tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg 1801 cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta 1861 tgttactaga tcgggaattc gatatcaagc ttggcactgg ccgtcgtttt acaacgtcgt 1921 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 1981 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 2041 aatggcgaat gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt 2101 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat 2161 tagaataacg gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg 2221 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct 2281 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca 2341 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt 2401 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca 2461 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga 2521 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca 2581 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg 2641 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca 2701 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg 2761 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg 2821 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg 2881 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga 2941 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga 3001 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg 3061 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac 3121 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac 3181 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt 3241 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg 3301 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt 3361 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca 3421 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc 3481 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg 3541 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa 3601 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc 3661 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg 3721 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc 3781 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa 3841 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag 3901 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac 3961 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc 4021 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta 4081 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca 4141 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc 4201 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca 4261 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa 4321 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc 4381 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc 4441 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga 4501 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga 4561 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg 4621 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc 4681 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc 4741 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg 4801 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca 4861 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact 4921 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa 4981 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga 5041 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt 5101 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga 5161 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga 5221 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct 5281 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct 5341 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg 5401 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc 5461 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt 5521 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca 5581 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga 5641 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa 5701 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa 5761 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc 5821 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg 5881 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc 5941 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc 6001 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg 6061 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 6121 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca 6181 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca 6241 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 6301 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 6361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 6421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 6481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 6541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 6601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 6661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 6721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 6781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 6841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 6901 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 6961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 7021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 7081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 7141 acgttaaggg attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata 7201 atattttatt ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata 7261 ctgttcttcc ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt 7321 gtccgccctg ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa 7381 gatgttgctg tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt 7441 ctttaaaaaa tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc 7501 gcaatccaca tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc 7561 taagctattc gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc 7621 cgcatacagc tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag 7681 gacgccatcg gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag 7741 gacctttgga acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac 7801 atcataggtg gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc 7861 tcccaccagc ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta 7921 tttttcgatc agttttttca attccggtga tattctcatt ttagccattt attatttcct 7981 tcctcttttc tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc 8041 aattcactgt tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg 8101 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca 8161 caggcagcaa cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt 8221 gtttcaaacc cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag 8281 tctgccgcct tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat 8341 cgagtggtga ttttgtgccg agctgccggt cggggagctg ttggctggct ggtggcagga 8401 tatattgtgg tgtaaacaaa ttgacgctta gacaacttaa taacacattg cggacgtttt 8461 taatgtactg aattaacgcc gaattaattc gagctcggat ctgataattt atttgaaaat 8521 tcataagaaa agcaaacgtt acatgaattg atgaaacaat acaaagacag ataaagccac 8581 gcacatttag gatattggcc gagattactg aatattgagt aagatcacgg aatttctgac 8641 aggagcatgt cttcaattca gcccaaatgg cagttgaaat actcaaaccg ccccatatgc 8701 aggagcggat cattcattgt ttgtttggtt gcctttgcca acatgggagt ccaagattct 8761 gcagttagat ctcggtgacg ggcaggaccg gacggggcgg taccggcagg ctgaagtcca 8821 gctgccagaa acccacgtca tgccagttcc cgtgcttgaa gccggccgcc cgcagcatgc 8881 cgcggggggc atatccgagc gcctcgtgca tgcgcacgct cgggtcgttg ggcagcccga 8941 tgacagcgac cacgctcttg aagccctgtg cctccaggga cttcagcagg tgggtgtaga 9001 gcgtggagcc cagtcccgtc cgctggtggc ggggggagac gtacacggtc gactcggccg 9061 tccagtcgta ggcgttgcgt gccttccagg ggcccgcgta ggcgatgccg gcgacctcgc 9121 cgtccacctc ggcgacgagc cagggatagc gctcccgcag acggacgagg tcgtccgtcc 9181 actcctgcgg ttcctgcggc tcggtacgga agttgaccgt gcttgtctcg atgtagtggt 9241 tgacgatggt gcagaccgcc ggcatgtccg cctcggtggc acggcggatg tcggccgggc 9301 gtcgttctgg gctcatcgat tcgatttggt gtatcgagat tggttatgaa attcagatgc 9361 tagtgtaatg tattggtaat ttgggaagat ataataggaa gcaaggctat ttatccattt 9421 ctgaaaaggc gaaatggcgt caccgcgagc gtcacgcgca ttccgttctt gctgtaaagc 9481 gttgtttggt acacttttga ctagcgaggc ttggcgtgtc agcgtatcta ttcaaaagtc 9541 gttaatggct gcggatcaag aaaaagttgg aatagaaaca gaatacccgc gaaattcagg 9601 cccggttgcc atgtcctaca cgccgaaata aacgaccaaa ttagtagaaa aataaaaact 9661 agctcagata cttacgtcac gtcttgcgca ctgatttgaa aaatctcaga attcgagct //