Andreas Nebenführ, Ph.D.

See Also: Nebenführ lab

 

Research Statement

The main focus of our lab is to study transport within plant cells. Transport processes can be expected to play an important role in setting up and maintaining the differential distribution of cellular components from proteins to organelles. The central objects of the research are myosin proteins in the model plant Arabidopsis thaliana. This class of motor proteins can move entire organelles through the cell and is also responsible for cytoplasmic streaming. These movements are likely to affect the functioning of organelles in the secretory system that have a constant exchange of membranes and proteins between them. As a second line of inquiry we are also studying these membrane transport processes, particularly to and from the Golgi apparatus. Our research involves cell-biological, biochemical, molecular, and genetic approaches, such as live-cell imaging, tissue culture, protein-protein interactions, mutant analysis, proteomics. Additional information can be found on the Nebenführ lab page.

Selected Publications

 

J.K. Vick, A. Nebenführ (2012) Putting on the breaks: Regulating organelle movements in plant cells. Journal of Integrative Plant Biology 54: 868-874.

S.L. Madison, A. Nebenführ (2011) Live-cell imaging of dual-labeled Golgi stacks in tobacco BY-2 cells reveals similar behaviors for different cisternae during movement and brefeldin A treatment. Molecular Plant 4: 896-908.

E. Park, A. Nebenführ (2011) Cytoskeleton and Root Hair Growth. in: The Plant Cytoskeleton (Ed. B. Liu) Advances in Plant Biology 2:259-275.

J.-F. Li, A. Nebenführ (2010) FAST Technique for Agrobacterium-Mediated Transient Gene Expression in Seedlings of Arabidopsis and Other Plant Species. Cold Spring Harbor Protocols doi:10.1101/pdb.prot5428

Li J, Nebenführ A (2008) Inter-dependence of dimerization and organelle binding in myosin XI. Plant Journal 55: 478-490.

Li J, Nebenführ A (2008) The tail that wags the dog: The globular tail domain defines the function of myosin V/XI. Traffic 9: 290-298.

Nebenführ A (2007) Organelle dynamics during cell division. in: Cell division control in plants. (Eds. Verma DPS and Hong Z). Plant Cell Monographs 9: 195-206.

Nelson B, Cai X, Nebenführ A (2007) A multi-color set of in vivo organelle markers for colocalization studies in Arabidopsis and other plants. Plant Journal 51: 1126-1136.

Li J, Nebenführ A (2007) Organelle targeting of myosin XI is mediated by two globular tail subdomains with separate binding sites. Journal of Biological Chemistry 282: 20593-20602. 

Saint-Jore-Dupas C, Nebenführ A, Boulaflous A, Follet-Gueye ML, Plasson C, Hawes C, Driouich A, Faye L, Gomord V (2006) Plant N-glycan processing enzymes employ different targeting mechanisms for their spatial arrangement along the secretory pathway. Plant Cell 18: 3182-3200. 

Subramanian C, Woo C, Cai X, Xu X, Servick S, Johnson CH, Nebenführ A, von Arnim AG (2006) A suite of tools and application notes for in vivo protein interaction assays using bioluminescence resonance energy transfer (BRET). Plant Journal 48: 138-152.

 

Contact Information

Office:
Room 240
Hesler Biology
Phone: (865) 974-9201

Lab:
Room 218
Hesler Biology
Phone: (865) 974-4927

Email: nebenfuehr@utk.edu